Denial-of-benefits clauses in investment treaties are generally designed to exclude treaty protection of nationals of third States which, through mailbox or shell companies, seek to benefit from the provisions that the State parties to the treaty did not intend to grant them. The specific meaning and application of relevant provisions of the international investment treaties is refined in the practice of international investment arbitration tribunals, thus the research on specific provisions must rely on international investment arbitration cases. This article examines denial-of-benefit clause invoked by Central Asian Countries and analyzes the content, application and procedural requirements of the clause. This article can provide reference for setting up the denial-of-benefits clause in the relevant international investment treaties of China and provide reference for the overseas investors with the use of this clause to deal with the host country in the court of arbitration for international investment.