Given the effect that cross-border railway transportation conforms to the development direction of the corridor, it becomes an important support for the construction of the trade corridor. The construction of the New International Land-sea Trade Corridor also provides a good opportunity for the development of cross-border railway transportation. However, it should be noted that some problems are likely to arise, such as the inconsistence of transport rules, the low degree of trade facilitation, the non-circulation of railway bills and the imperfect dispute settlement mechanism, which restricts the construction of the corridor. Therefore, China needs to improve the legal guarantee system of cross-border railway transportation, enhance the trade facilitation degree of the countries along the corridor, explore the property right of the railway bill, and construct the diversified dispute settlement mechanism.